Topflash/fopflash reporter assay
Web1. aug 2024 · From what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt … Webtopflash luciferase reporter plasmid - by Bioz Stars , 2024-03 86 / 100 stars Images 1) Product Images from "Insulin-like growth factor 2 mRNA-binding protein 3 promotes kidney injury by regulating β -catenin signaling" Article Title: Insulin-like growth factor 2 mRNA-binding protein 3 promotes kidney injury by regulating β -catenin signaling
Topflash/fopflash reporter assay
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WebCell-based assays using synthetic TCF/LEF (T-cell factor/lymphoid enhancer factor) reporters, as readouts of β-catenin/TCF-dependent transcriptional activity, have contributed greatly to the discovery of small molecules that modulate Wnt signaling. In the present study, we report a novel screening method, called a bidirectional dual reporter ... Web一般而言，topflash/fopflash是比较常用的检测β-catenin的系统，原理和所有的RLU一样，都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基因的表达。 如 …
WebIn the present study, we report a novel screening method, called a bidirectional dual reporter assay. Integrated transcriptome analysis identified a histidine ammonia-lyase gene (HAL) … WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites. ...
Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a … Web14. máj 2024 · The TOPFlash/FOPFlash luciferase reporter assays demonstrated that sulfono-γ-AApeptides can selectively suppress transactivation of Wnt/β−catenin …
Webfopflash plasmids - by Bioz Stars , 2024-03 94 / 100 stars Images 1) Product Images from "Methylation and Loss of Secreted Frizzled-Related Protein 3 Enhances Melanoma Cell Migration and Invasion" Article Title: Methylation and Loss of Secreted Frizzled-Related Protein 3 Enhances Melanoma Cell Migration and Invasion Journal: PLoS ONE
WebFrom that 50ng DNA, 15ng are TOPFlash plasmid (Firefly), 10 ng Renilla, and the rest empty plasmid (PCS2 or pCDNA3). Treat the cells O/N with concentrations between 1-3uM of … heat index in omaha neWebFigure Legend Snippet: (a) TCF/LEF luciferase reporter assay in A549 cells. To characterize the sLRP6E1E2 effects on the Wnt3a/β-catenin signaling, cells were transfected with … heat index in miami todayWebThis reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is … heat index in manila nowWeb本人近日做TOPflash实验检测wnt通路的激活情况，遇到些许问题，希望做过TOPflash assay或了解wnt通路的战友解疑答惑。. 1、看了些相关文献，既有分别转染TOPflash … movies to watch before avengers endgameWebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from … heat index in philippinesWebDual Luciferase (Firefly-Renilla) Assay System (BPS Bioscience, #60683): This system assays cells directly in growth medium. It can be used with any luminometer. Automated injectors are not required. • Luminometer Concentration Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each) Background heat index in nashvilleWebThe TopFlash assay is a luciferase reporter assay and it pot be used to monitor the concentration of both Wnt and R-spondin in conditioned media The assay begins when 2 luciferase expressing plasmids one of inducible and name other of constitutive expression are transfected into HEK293 cells. In wnt reporter assays were measured the topflash. movies to watch before avengers infinity war