Topflash/fopflash assay
WebTOPflash reporter assay demonstrates a significant decrease in catenin transcriptional activity in Hep3B and HepG2 cells following -catenin siRNA transfection for 48 hours (P < … WebThe assay uses a TOPFlash luciferase reporter which has binding sites for. TCF Reporter Plasmid Kit four of transfection grade T cell factor TCF reporter plasmids for over in TOPFlash and FOPFlash wntb-catenin activity assays. -CateninLEF1 transactivates the microRNA-371-373 cluster. Ecm from promega kit from the topflash reporter assay ...
Topflash/fopflash assay
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WebTCF Reporter Plasmid Kit Set of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays.; Synonyms: TOPFlash/FOPFlash, TOPFlash plasmid, FOPFlash plasmid; find Sigma-Aldrich-17285 MSDS, related peer-reviewed papers, technical documents, similar products & more at … WebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay was …
WebJ-STAGE Home Web17. sep 2024 · The activity of osteogenic differentiation was evaluated with alizarin red staining, alkaline phosphatase activity assay, and RT-qPCR. Wnt/β-catenin signaling activities were compared among groups by TOPFlash/FOPFlash assay, immunofluorescence staining, and western blot assay of beta-catenin (CTNNB1). Coimmunoprecipitation mass …
WebThen, we performed TOPFlash/FOPFlash assay to examine the activity of Wnt/β-catenin signaling. After verification of miR-100-5p overexpression in TPC-1 and KTC-1 by transfection of miR-100-5p mimic (), miR-100-5p mimic was observed to decrease TOPFlash luciferase in TPC-1 and KTC-1 in a dose-dependent manner (Figure 2c and d).Moreover, … WebUpon TOPFLASH analysis, Cby-induced suppression of reporter activity was augmented significantly in HEK293 cells as TC1-siRNA was increased to 10 nmol/L (P < 0.004, …
Web21. feb 2012 · TOPflash and FOPflash constructs are widely used to evaluate β-catenin-dependent signaling events that drive the expression of TCF. TOPflash is a TCF reporter plasmid containing two sets of three copies of wild-type TCF binding sites driven by the thymidine kinase minimal prompter and upstream of a luciferase reporter gene.
WebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from … horowhenua mail contactWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : … horowhenua netball websiteWebUpstate Biotechnology Inc topflash fopflash luciferase reporter constructs Topflash Fopflash Luciferase Reporter Constructs, supplied by Upstate Biotechnology Inc, used in … horowhenua mail onlineWebPlasmid M50 Super 8x TOPFlash from Dr. Randall Moon's lab contains the insert TCF/LEF binding sites and is published in Curr Biol. 2003 Apr 15. 13(8):680-5. This plasmid is available through Addgene. ... M51 Super 8x FOPFlash (TOPFlash mutant) human beta-catenin pcDNA3 pRL-SV40P ... horowhenua latest newsWebDescription. FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three … horowhenua kamarWeb4. dec 2024 · Wnt signaling activity was observed using a Top/Fopflash assay, real-time quantitative RT-PCR, immunoblotting, immunofluorescence staining, and chromatin immunoprecipitation. Results CircAXIN1 is highly expressed in GC tissues compared with its expression in paired adjacent normal gastric tissues. horowhenua joinery and kitchensWebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold … horowhenua mayor