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Scrape loading dye transfer assay

WebA scrape loading/dye transfer assay was used to assess the effects of the chemicals on GJIC. In this assay, a scratch is made in a confluent cell layer in the presence of a Lucifer Yellow (LY) solution. This fluorescent dye is taken up by damaged cells along the scrape and is passed through to the neighboring cells when they display functional ... WebOne of these assays, the scrape-loading dye transfer (SLIDT) technique, is based on monitoring the transfer of the fluorescent dye Lucifer yellow from one cell into adjacent cells via functional gap junctions. The objective of our study was to evaluate and compare various approaches for quantifying results obtained with the SL/DT technique.

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WebMar 7, 2024 · Scrape-loading dye transfer in vitro. Gap junction-mediated intercellular coupling was evaluated by transfer of lucifer yellow dye in a scrape-loading assay as described previously with minor modifications . Briefly, astrocytes were seeded on 12-well culture plates and incubated at 37 °C and 5% humidified atmosphere. WebVoltage is an important parameter that regulates the open probability of both intercellular channels (gap junctions) and undocked hemichannels formed by members of the connexin gene family. All ... clock braunアナログ時計 スクリーンセーバー https://adoptiondiscussions.com

Scrape Loading/Dye Transfer Assay - Springer Nature

WebFigure 7 Utility of the scrape loading/dye transfer assay to detect a toxin/toxicant s ability to modulate gap junctional intercellular communication. The last two images (6ngmr TPA) illustrate both the epifluorescent and phase images, respectively, of the same cells to show that the complete inhibition of GJIC was not associated with the death ... WebJul 1, 2008 · The scrape loading and dye transfer (SL/DT) method is a functional assay widely used to evaluate the level of the intercellular communication via gap junction. … WebFeb 28, 2011 · An innovative adaptation of traditional, fluorescence microscopy-based scrape loading-dye transfer (SL-DT) assay is presented, which has been optimized to simultaneously evaluate GJIC, cell density and viability, which could be included into in vitro screening and assessment of pharmacologically and toxicologically relevant compounds. … clock braunアナログ時計

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Scrape loading dye transfer assay

The caspase-1 inhibitor VX765 upregulates connexin 43 ... - Nature

WebSep 17, 2024 · The scrape loading/dye transfer assay was employed to access lucifer Yellow molecules transfer efficiency mediated by cell-cell communication. Connexin43 (Cx43), Pannexin1 (Panx1), MAPK, and the PI3K/Akt signaling pathway proteins were examined via Western blotting. Immunofluorescence was applied to visualize the … WebMay 21, 2016 · The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically … For over 35 years, biological scientists have come to rely on the research protocols … Scrape Loading/Dye Transfer Assay. Pavel Babica, Iva Sovadinová, Brad L. Upham; …

Scrape loading dye transfer assay

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WebSep 3, 2024 · MC3T3-E1 cell line were cultured in a 25 cm2flask containing 3-4 ml α-MEM with 10% fetal bovine serum at 37 °C in a humidified atmosphere of 5% CO2in the air. FGF7 treatment Both primary osteoblasts and MC3T3-E1 cell line were seeded onto six-well plates at 5 × 105cells per well (85%~95% confluence). WebJan 20, 2024 · Fluoranthene as a non-cytotoxic GJIC inhibitor assessed using the multiparametric scrape-loading-dye transfer (SL-DT) assay in rat liver progenitor WB-F344 cells (a 24-well plate format, 1-h exposure). (a) The effects were compared with the non-treated control and expressed as the fraction of control (FOC; FOC non-treated control = …

WebScrape loading, Tracers 1 Introduction Dye coupling methods are by far the most frequently used assay for the assessment of GJIC, mainly because of their ease of use. Of all the … WebFeb 1, 1987 · It employs scrape-loading to introduce a low molecular weight (MW) fluorescent dye, Lucifer yellow CH (MW 457.2) into cells in culture and allows the …

WebFeb 7, 2024 · e Images obtained from the scrape loading (SL)/dye transfer (DT) assay after application of lucifer yellow CH dilithium salt (LY, MW 457), which transfers via the functional gap junction channels ...

Webin scrape-loading dye transfer assay [ 13] Packaging 25, 100 mg in glass bottle Bottomless glass bottle. Contents are inside inserted fused cone. Features and Benefits This …

WebAug 20, 2024 · Among various techniques developed for in vitro assessment of GJIC, the SL-DT (i.e., scrape loading-dye transfer) assay has probably been most frequently used in multiple studies of toxicant or carcinogen effects on GJIC. This in vitro assay is applicable to various cell types and cell lines. clockology fans ダウンロードWebMay 21, 2016 · Scrape-load dye-transfer assay was performed as previously described using 5 minutes room temperature incubation time post-scrape. 57 Cells were washed three … cloche 服 ブランドWebThe GJIC was investigated by a scrape-loading dye transfer assay. Expression of Cx43 and K3 keratin as well as BrdU-retaining nuclei were also ana-lyzed after xenotransplantation in nude mice. Results: Limbal epithelium showed mean±SD 12.4% ±14.5%positiveunitsof Cx43expressionandalowBrdU clockology fans ダウンロード出来ないWebDec 22, 2024 · The scrape-loading dye transfer assay adapted from (Babica et al. 2016) was used to assess gap-junction intercellular communication within an EB. A clean linear cut was made across an individual EB plated on a glass coverslip using a curved surgical scalpel blade. The EB was then loaded with lucifer yellow fluorescent dye added into the culture ... clocklogy ダウンロード エルメスWebThe scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large … clockology おすすめ文字盤WebJan 24, 2013 · The function of cells coupling was measured by Scrap loading dye transfer assay. Our results showed that the AGE-infused rat hearts exhibited increased cardiac RAGE and Cx43, as well as Cx43 redistribution. In cultured cardiomyocytes, AGE elevated RAGE expression in a time- and dose-dependent manner. Cx43 protein and mRNA levels were ... clockology おすすめWebJul 31, 2024 · A scrape loading/dye transfer assay was performed to assess the GJIC function. The expression of connexins was examined by real-time reverse transcription polymerase chain reaction (RT-PCR), western blot and immunofluorescent staining. clockup sentinel -センチネル- hdリマスター