WebJul 10, 2014 · The phospho-independent UPF1 interaction motif of SMG6 localized to a stretch that is proximal to the TPR domain and distinct from the EBMs located within the first 160 residues. (D) GST pull-down ... WebProtein phosphatase 1 (PP1) belongs to a certain class of phosphatases known as protein serine/threonine phosphatases.This type of phosphatase includes metal-dependent …
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WebMay 29, 2010 · Compared with WT, GT tMEFs showed reduced levels of an anti–phospho-S/T-Q antibody–detected band comigrating with Upf1 in both the lysate and anti-Upf1 immunoprecipitate (Fig. 1E). This result confirms that disruption of Smg1 expression in Smg1 gt/gt embryos reduces the phosphorylation levels of Upf1. WebPhospho-dependent and phospho-independent interactions of the helicase UPF1 with the NMD factors SMG5–SMG7 and SMG6 Sutapa Chakrabarti, Sutapa Chakrabarti Max …
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WebJul 3, 2024 · UPF1 overexpression protects neurons from R-DPR toxicity Consistent with the notion that R-DPRs cause neurodegeneration in part through inhibiting NMD, recent studies have shown that... WebFeb 21, 2006 · A fraction of hyperphosphorylated UPF1 associates with chromatin of unperturbed cells, and chromatin association increases in S phase and upon γ irradiation. ATR phosphorylates UPF1 both in vitro and in vivo, and shRNA-mediated downregulation of ATR diminished the association of UPF1 with chromatin, although it did not affect NMD.
WebPan Phospho-Tyrosine (2) Phospho Y705 (1) MonoMethyl K4 (1) Phospho S9 (1) DiMethyl K9 (1) DiMethyl K4 (2) Unconjugated (11) Phospho Y15 (1) TriMethyl K36 (2) Phospho S15 (1) ... UPF1 UPF1; DNMT1 DNMT1; FURIN FURIN; ACLY ACLY; ALDH1A1 ALDH1A1; PROM1 PROM1; MCAM MCAM; CD62P CD62P; P-SELECTIN P-SELECTIN; CALR CALR; CLTC …
WebAnti-phospho-Upf1 (Ser1127), Cat. No. 07-1016, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 1 (RENT1) phosphorylated at Ser1127 and has been tested in Immunoprecipitation, peptide … rale snowbikeWebApr 18, 2008 · We provide evidence that phospho-Upf1 functions after nonsense codon recognition during steps that involve the translation initiation factor eIF3 and mRNA decay … raleigh\u0027s pubWebMay 18, 2024 · In certain embodiments, the SMG1-marker of the methods described herein comprises two or more of phospho-UPF1, LUC7L, NEU1, SRSF3, SRSF6, HNRNPL, HNRNPK, RPS12, or SF1. In certain embodiments, the SMG1-marker of the methods described herein comprises at least 3, 4, 5, or all of phospho-UPF1, LUC7L, NEU1, SRSF3, SRSF6, HNRNPL, … raley\\u0027s arizonaWebDec 14, 2024 · While preclearing each lysate, prepare the antibody−Dynabeads Protein A (see Note 10) by adding 5–10 μg of anti-phospho-UPF1 (Ser1116) antibody or, as a negative control, rabbit IgG (see Note 11) to 50 μl of washed Dynabeads Protein A from Subheading 3.3, step 1 in 300 μl of Hypotonic Gentle Lysis Buffer containing 150 mM NaCl (see Note … dr ilic ginekolog dragisa misovicWebJan 28, 2013 · Phospho-Upf1 also recruits the SMG-6 endonuclease which might be involved in endo-cleavage. Upf1 ATPase/helicase activities are likely required for the … dr. ilia poliakovWebJul 3, 2024 · Phospho-UPF1 (p-UPF1) is a prominent marker of NMD substrates as its presence commits an RNA to degradation 17, 18, 41, 42. To identify NMD substrates in PEL cells we performed fRIP-coupled to... raleigh\u0027s salveWebUpf1 is a phosphoprotein ( Pal et al., 2001 ), and Upf1 phosphorylation has been shown to influence Upf1 binding to SMG-5, SMG-7, protein phosphatase 2A, Upf2, and distinct isoforms of Upf3 ( Kashima et al., 2006 , Ohnishi et al., 2003 ). Upf1 phosphorylation by SMG-1 is essential for NMD ( Brumbaugh et al., 2004 , Yamashita et al., 2001 dr iliaeva köln