WebThis micro-PEG assay is used to evaluate various broadly neutralizing mAb candidates to HIV-1 viral spike (gp120 and gp41 glycoproteins). Using ∼1 mg of VRC01-WT mAb per assay, the precision of the micro-PEG assay was established. A series of 7 different broadly neutralizing mAbs to the HIV-1 viral spike proteins were compared by curve shape ... WebNov 1, 2013 · The PEG-switch assay has been successfully evaluated for two known redox sensitive kinases in rat cardiac tissue. This novel method involves fewer steps than …
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WebJul 12, 2013 · The PEG-switch assay is a fast effective semi-quantitative method for measuring target reversible cysteine oxidation in complex protein mixtures derived from … WebFigure 3 Gel shift assay mechanism by PEG-PCMal depend on protein redox state. Developer: Dojindo Molecular Technologies, Inc. Manual. Manual. ... “The PEG-switch assay: A fast semi-quantitative method to determine protein reversible cysteine oxidation”, J Pharmacol Toxicol Methods., 2013, 68, (3), 297. 4. L. JTetsch, C. Koller, A. Donhofer ... destiny 2 hawkmoon or dead man\\u0027s tale
The PEG-switch assay: a fast semi-quantitative method to ... - CNGB
Webveloped a mass-tag labeling method termed acyl-PEG exchange (APE) that exploits the NH 2OH-sensitivity of thioesters and selective reactivity of Cys residues for site-specific … WebAug 10, 2015 · A recent variation on the biotin-switch assay is labeling oxidized thiols with maleimide conjugated to a 5 kDa PEG-tag (Burgoyne et al., 2013). This allows simple and rapid determination of target oxidation without the need for affinity purification. If a protein is oxidized it will be labeled following reduction with PEG-maleimide causing it ... destiny 2 hawkmoon god rolls