Pachlt-c
WebpAcHLT-A, was also digested withEcoRI and NotI, and the product was purified by gel extraction following electro-phoresis on agarose. The ligation of PLC (5 kbp) and baculovirus transfer vector pAcHLT-A was carried out for 18hat16°C using T4 DNA ligase. The reaction mixture contained 1 íLofNotI and EcoRI digested pAcHLT-A, 8 WebSep 14, 2010 · into baculovirus vector, pAcHLT-C (Pharmingen) via PCR amplifi-cation at XhoI and NcoI sites. Cell culture. Human embryonic kidney (HEK)293, HEK293T, and ... methanol at 4°C at 40 V overnight, and the membranes were incu-bated in TBST (containing 100 mM Tris-Cl, pH 7.5, 150 mM NaCl,
Pachlt-c
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WebABSTRACT: This paper uses phospholipase C as a model to demonstrate that lipids can act as ligands to bind to specific motifs and regulate protein activity via allosteric effects. … WebThe BRD4 ΔN (amino acids 699–1,400) was generated as a dropout mutant in the pAcHLT-C baculovirus transfer vector from the Flag- BRD4 WT. The plasmid bearing GST-tagged human BRD4 in the pGEX-6P-1 bacterial expression vector was obtained from Addgene (plasmid 14447). The human Flag-tagged BRD4 WT and FAA-AAA mutant in the pCDNA …
WebMay 1, 2004 · Cotransfection of Spodoptera frugiperda 9 cells (BD PharMingen) with linearized BaculoGold baculovirus DNA (BD PharMingen) and pAcHLT-C/pfgl2 generated a recombinant baculovirus encoding pfgl2 protein with a polyhistidine (His) tag. High Five (H5) cells (Invitrogen), used for high-yield protein production, were infected with baculovirus at … WebpAcHLT-C Baculovirus Transfer Vector: His tag: INQUIRY: TVC2009 : pAcMP2, pAcMP3 Baculovirus Transfer Vector Set: N: INQUIRY: TVC2010 : pAcMP2 Baculovirus Transfer Vector Set ... pAcGP67 A,B,C Baculovirus Transfer Vector Set : gp67 signal sequence: INQUIRY: TVC2024
WebApr 30, 1999 · BIG2-Full, excised with Nde I and Not I, was ligated to the baculovirus transfer vector pAcHLT-C with the His 6 sequence encoded at its NH 2 terminus. A sample (2 μg) and 0.5 μg of BaculoGold DNA (Pharmingen) were mixed with 2 × 10 6 Sf9 cells in 3 ml of TNM-FH Insect medium (Pharmingen).
WebpAcHLT-A, -B, -C Polyhedrin very late yes 6xHis-tag, thrombin cleavage site pAcHLT-A-GFP/BFP/YP Polyhedrin very late yes GFP tag, 6xHis tag, thrombin ... C Polyhedrin very …
WebEach school year PAL Youth must complete 2 steps BEFORE joining any of our programs. The membership for 2024-23 includes Afternoon Enrichment, Summer Camp 2024 and all … tax document for sale of homeWebThe resources, processes, procedures, design considerations, and methods to ensure that all system, equipment, and support items are preserved, packaged, handled, and transported … the cherub pub dartmouthWebThe two fragments were ligated into pAcHLT-C, which had been digested with NdeI and NotI. DCD570 was amplified from DC plasmid by polymerase chain reaction using forward primer 59-AAA-CATATGTTGTGACTTAAATG (NdeI site underlined) and reverse primer G54. The amplified DNA was first subcloned into pCRscript the cherubim and seraphimWebvirus transfer vector (pAcHLT-C). DCD520 was made similarly by liga-tion of two DNA fragments using the SpeI site. One fragment was amplified from plasmid DC using … the cherub seriesWebOnline Inquiry Home; Products. Cell Product; Expression Related Kit; Medium Product; Vector Product; Services. Bacterial Cell Protein Expression Services tax documents for filing taxesWebpAcHLT-A, -B, -C Polyhedrin very late yes 6xHis-tag, thrombin cleavage site pAcHLT-A-GFP/BFP/YP Polyhedrin very late yes GFP tag, 6xHis tag, thrombin ... C Polyhedrin very late yes Signal peptide pAcSecG2T Polyhedrin very late yes Signal peptide, GST-tag pBACsurf-1 Polyhedrin very late no gp64 signal peptide, gp64 mature ... tax documents for tspWebSegment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into … the cherub school