WitrynaIn this GUI, CTF estimation and correction are done using CTFFIND4 and IMOD's ctfphaseflip. This part will not be used in the workshop. Reconstruction Binned reconstruction. Click on binned reconstruction to create a binned tomogram using the previously created binned aligned tilt series. Adapt the tomogram height if necessary … Witrynactfphaseflip(1) General Commands Manual ctfphaseflip(1) NAME ctfphaseflip ... A sample command file for running the program with "subm" can be found in the …
The prefusion structure of herpes simplex virus glycoprotein B
Witryna25 wrz 2024 · Defocus estimation was performed using IMOD ctfplotter , phase flipping was performed using NovaCTF or ctfphaseflip, and dose weighting was performed using IMOD mtffilter. Tomograms were aligned using IMOD etomo and subsequently reconstructed using NovaCTF, with the exception of eight tomograms that were used … Witryna1 lip 2012 · Local approaches, however, are currently the standard in the field. For example the program CTFPHASEFLIP [6] as incorporated in the widely used software package IMOD [16] ... Projection after (local) tilted phase flipping using CTFPHASEFLIP, which is part of IMOD. (D) Difference between (B) and (C). The tilt-axis is horizontal. … touch screen magic touch
Direct Cryo-ET observation of platelet deformation induced by …
Witryna5 lis 2024 · Tomograms were reconstructed in IMOD using weighted back-projection, with a simultaneous iterative reconstruction technique (SIRT)–like filter to aid visualization and segmentation. Defocus-contrast tomograms were contrast transfer function corrected in IMOD using ctfphaseflip. VPP tomograms were left uncorrected. Witryna4/2/10: Fixed initialization problem in Ctfphaseflip's detection of bad view numbers and fixed the warning when a simple one-line file is used. 4/1/10: Fixed bug in Ctfplotter … Witryna1 paź 2009 · The CTF was estimated using GCTF 61 and then the IMOD ctfphaseflip implementation was used for phase correction 62. B Nearest neighbor distance … touch screen magnification