Hitrap desalting说明书
Web11 HiTrap Desalting, HiPrep 26/10 Desalting or PD-10 Desalting columns if necessary (Table 5). Note: The reuse of HiTrap rProtein A FF depends on the nature of the sample and should only be performed with identical monoclonals to prevent cross-contamination. 10 … WebTable 4.Prepacked columns for desalting and buffer exchange Column Loading volume Elution volume HiPrep™ 26/10 Desalting1 2.5 to 15 mL 7.5 to 20 mL HiTrap Desalting2 …
Hitrap desalting说明书
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Web产品支持. HiTrap 脱盐柱是即用型 5 mL 层析柱，预装有 Sephadex G-25 Superfine 层析填料，可快速便利地进行脱盐和缓冲液置换。. 脱盐及缓冲液置换便利，适用于 1.5 mL 及以 … WebDesalting. In group separation (desalting) the sample volumes can be up to 30% of the column volume. Regarding sample volumes for prepacked columns, please refer to …
Web11 lug 2010 · Keep flowrate range1–10 ml/min. Collect fractions. approximately10 ml buffer before applying nextsample. Collect fractions. Note: sampleinjection tubingsample loops … WebFor desalting and buffer exchange of protein extracts (M r > 5000). HiTrap Desalting 17-1408-01 0.25–1.5 ml 1.0–2.0 ml Prepacked with Sephadex G-25 Superfine. Requires a syringe or pump to run. PD-10 Desalting 17-0851-01 1.0–2.5 ml 1 1.75–2.5 ml 2 3.5 ml 1 Up to 2.5 ml 2 Prepacked with Sephadex G-25 Medium. Runs by gravity flow or ...
WebHiTrap™ Desalting 5 × 5 ml 17-1408-01 HiTrap Desalting 100 × 5 ml1 11-0003-29 1 Special pack size delivered on specific customer order. Accessories To connect columns … WebHiTrap ® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex ® G-25 Superfine, for fast and convenient desalting and buffer exchange. HiTrap ® Desalting …
WebThe effect of different sample volumes on the HiTrap Desalting column using tubing loops for sample injection. Column: HiTrap Desalting Sample: 2 mg/ml bovine serum albumin …
WebHiTrap Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction. Application python selenium xpath childWebSuggested remedy. Column is not clean enough. 1. Reverse the flow and pump 200 ml of buffer through the column at a flow rate of 15 ml/min. 2. Clean the column according to instructions. If the pressure is not decreased reverse the flow direction again and follow the more rigorous cleaning instructions. python selenium-wireWebHiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange. Convenient desalting and … python selenium xpath linkWebThe effect of different sample volu mes on the HiTrap Desalting column using a syringe for sample injection Column: HiTrap Desalting Sample: 2 mg/ml bovine serum albumin in … python self nedirWeb• Exchange buffer using a Prepacked columns for desalting, refer to Table 4. 2 Filter the sample through a 0.45 μm filter or centrifuge immediately before loading it to the ... HiPrep™ 26/10 Desalting1 2.5 to 15 mL 7.5 to 20 mL HiTrap Desalting2 0.25 to 1.5 mL 1.0 to 2.0 mL PD-10 Desalting3 1.0 to 2.5 mL4 1.75 to 2.5 mL5 3.5 mL Up to 2.5 ... python self co toWebThe idea of the desalting step is to purify my labeled compound from un-attached nuclide, but even if I start with an already purified version (from gravity, NAP5, since this works … python self x yWebHiTrap 1 mL: 4 mL/min HiTrap 5 mL: 20 mL/min Recommended operating flow rate HiTrap 1 mL: 1 mL/min HiTrap 5 mL: 5 mL/min Chemical stability Stable to commonly used … python self._name