Flp-in cho
WebJan 13, 2024 · HEK & CHO mammalian cell lines in comparison. CHO mammalian cell lines have, similar to HEK cells, a long history of being used for the production of various … WebFlp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster …
Flp-in cho
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WebA single gene copy of shRNA-2 also decreased the translational level of DHFR by 80% in Flp-In CHO cells. shRNA-2 was then incorporated into a plasmid vector expressing human erythropoietin (EPO) and an exogenous DHFR to develop … WebJan 15, 2024 · We also established CHO cells expressing the WT protein using the Flp-In system as previously described (thereafter called Flp-In CHO-WT cells) [6]. The pcDNA5/FRT-PiT-2-flag in GFP vectors were obtained by inserting GFP into Loop7 of the SLC20A2 (WT, T115 M, and R467X) using in-fusion cloning system (Takara Bio Inc.; …
WebLike Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage. In FLP-FRT, FLP is analogous to cre and FRT to loxP – FLP recognizes the FRT sites, and catalyzes recombination between them. WebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably …
WebAug 8, 2016 · MIL75-pFRT-FTMK and POG44 were co-transfected (1:9) into Flp-In-CHO cells. After 48 h of transfection, the cells were cultured under selection pressure by hygromycin B (1 mg/ml). WebThe Flp-In-CHO Cell Line was created by transfecting CHO cells with pFRT/ lac Zeo2 and selecting for Zeocin™-resistant clones Flp-In-CHO cell lines work well with Flp-In vectors that express gene from CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™, and pSecTag/FRT/V5-His-TOPO™)
WebFeb 13, 2024 · Flp‐In CHO‐METKO cells (METKO), wild‐type MET (MET WT)–, or 11N all 11Q mutant MET (11N all 11Q)–transfected Flp‐In CHO‐METKO cells were stained with FITC‐conjugated anti‐MET antibody and analyzed by flow cytometry. C, Confocal microscopy analysis of MET (red) and nuclear (cyan). Flp‐In CHO‐METKO cells …
WebAll of the Flp-In ™ cell lines (except Flp-In -CHO; see the following section) contain a single integrated FRT site and stably express the lacZ-Zeocin™ fusion gene from the pFRT/lacZeo plasmid under the control of the SV40 early promoter (see the following diagram). The location of the FRT site in each pink flower stemsWebFlp-In™ T-REx™-293 3 × 106 cells, frozen R780-07 Flp-In™ Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™ host cell lines that stably express the lacZ-Zeocin™ fusion gene from pFRT/lacZeo or pFRT/lacZeo2 (Flp-In™-CHO). Each cell line contains a single integrated FRT site steam wont update my gameWebA, assay for the evaluation of the interaction between cells and surface-bound collectins. Step 1: incubation of AF 488-conjugated bioparticles with collectins. Step 2: incubation of … steam won\u0027t recognize ps5 controllerWebThe Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/ lac Zeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains … pink flower starts with cWebDec 1, 2024 · Flp-In-CHO cells (Life Technologies) were maintained in F12 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco) at 37 °C in a humidified incubator containing 5% CO 2. The cells were transfected with 1 μg plasmid (pDF-λ1:pOG44 was 1:9) using Lipo 2000 (Life Technologies) according to the … steam won\u0027t purchase a gameWebAug 24, 2024 · Stable Flp-In™ CHO expression cell lines were generated by co-transfection of Flp-In™ expression construct (pcDNA™5/FRT/TO_FSHR) and the pOG44 plasmid (Invitrogen #V6005-20). Co-transfection was done using lipid-mediated transfection (Lipofectamine; Invitrogen #11668, Thermo Fisher Scientific Inc., Waltham, MA, USA … pink flower stained glassWebOct 25, 2024 · Flp-In-CHO:huOX40 cells as well as Flp-In-CHO:vector cells generated in-house (Pieris) were cultivated in Ham’s F12 medium (Gibco, #31765-027) with 10% FBS and 500 µg/ml hygromycin B. All mammalian cell lines were grown at 37°C and 5% CO 2. Goat anti-rabbit IgG-PE conjugate was purchased from Dianova/Jackson … steam won\u0027t start up