WebAdd 1/10 volume reducing agent (stabilized DTT) or use your own fresh 0.5 mM DTT. Heat 10 min at 70 degrees (boiling for 2-3 min probably works as well). 2 2. Load samples to NuPAGE gel and run as outlined above. The gels are 1.0 mM thick and the 15 well comb can hold a total of 15 WebInvitrogen™ 10X Bolt™ Sample Reducing Agent Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats. Brand: ... Sample Reducing Agent (10X) contains 500 mM dithiothreitol (DTT). Store at 2°C to 8°C. For Use With (Equipment) Bolt: Form: Liquid: Gel Compatibility: Bolt™ Bis-Tris Plus Gels:

10X Bolt™ Sample Reducing Agent - Thermo Fisher Scientific

WebBolt Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitol (DTT) for a 10X concentration in a stabilized liquid form. Contents. Sample Reducing Agent (10X) contains 500 mM dithiothreitol (DTT). More WebMar 2, 2024 · Add 2 µL 10X reducing agent to each sample. Spin the sample briefly in the microcentrifuge. Heat the samples for 10 min at 100 °C in a heat block. Spin the sample briefly in the microcentrifuge. Remove the gel from the plastic wrapper and rinse with deionized water. Gently remove the white sticker on the bottom of the gel. bluegreen vacation suites hershey park

10X Bolt™ Sample Reducing Agent - thermofisher.com

WebNuPAGE Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitol (DTT) for a 10X concentration in a stabilized liquid form. See all available buffers … WebBolt buffers and sample reducing agents are optimized for use with Bolt Bis-Tris Plus gels, and are available in a variety of formats. Note: Bolt gel run times may vary depending … WebBolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the … blue green wall colors